Transgenesis Techniques: Principles And Protocols Elizabeth J. Cartwright

ISBN: 9781603270182

Published: June 1st 2009

Hardcover

335 pages


Description

Transgenesis Techniques: Principles And Protocols  by  Elizabeth J. Cartwright

Transgenesis Techniques: Principles And Protocols by Elizabeth J. Cartwright
June 1st 2009 | Hardcover | PDF, EPUB, FB2, DjVu, AUDIO, mp3, RTF | 335 pages | ISBN: 9781603270182 | 3.39 Mb

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates onMoreThis revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection.

Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.



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